Full article: The development and hepatotoxicity of acetaminophen: reviewing over a century of progress Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. About Acetaminophen Overdose